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« A Terapia com Laser de Baixa Intensidade (LBI) pode Reduzir o Edema Pulmonar, tanto o influxo de Neutrófilos quanto o Nível de IL-1B nas vias Aéreas Pós Inflamação Induzida por LPS no Pulmão
Terapia co Laser em Baixa Intensidade no Tratamento da Síndrome de Ardência Bucal - Relato de Caso »

Nova Possibilidade de Sinergismo em Terapia com Laser de Baixa Intensidade (LBI) Associada ao Anti-Oxidante (N-acetil cisteina) Reduzindo Sintomas Precoces e Tardios em Vias Aéreas sobre a Expressão de Proteína Inflamatória de Macrófago (MIP-2) e Expressão de mRNA com a Geração de Espécies Reativas de Oxigênio (EROs)

F. Mafra de Lima1A. Balbin Villaverde2R. Albertini2,A. P. Ligeiro de Oliveira3H. C. Castro-Faria-Neto4*F. Aimbire21 Instituto de Pesquisa e Desenvolvimento, IP&D, UNIVAP, São Josédos Campos, CEP: 12244-000, São Paulo - SP - Brazil.2 Instituto de Engenharia Biomédica - Universidade Camilo Castelo Branco- UNICASTELO, Rodovia Presidente Dutra, Km 138, São José dosCampos, CEP: 12247-004 - São Paulo - SP - Brazil.3 Institute of Biomedical Science, Department of Pharmacology, Av.Prof. Lineu Prestes, 1524, CEP: 05508-900 - São Paulo - SP - Brazil.4 Laboratory of Immunopharmacology, IOC, FIOCRUZ, Po Box 926,CEP: 21045-900 - Rio de Janeiro - RJ - Brazil.Endereço:Flávio Aimbire. Biomedical Engineering Institute. Universidade Camilo Castelo Branco - UNICASTELO, Km 138, Rodovia PresidenteDutra Km 138 - São José dos Campos - CEP: 12247-004 - São Paulo - SP - Brazil. E-mail: flavio.aimbire@unicastelo.brAbstractObjective: The aim of this work was to investigate the LowLevel Laser Therapy (LLLT) effect on alveolar macrophages (AM)activated by oxidative stress and lipopolysaccharide (LPS).Background data: LLLT has been reported to actuate positivelyfor relieving the late and early symptoms of airway and lunginflammation. It is not known if the increased MIP-2 mRNAexpression and intracellular ROS generation observed in acute lunginflammation (ALI) can be influenced by LLLT. Methods: Rat AMcell line, AMJ2-C11 was cultured in a medium with LPS or H2O2and Laser irradiated (ë= 660 nm) at Fluence of 4.5 J/cm2. MIP-2mRNA and ROS production in AM were evaluated by Real Time-PCR and the 2′,7′-dichlorofluorescin diacetate (DCFH-DA),respectively. The NF-êB protein in AM was measured by ELISAResumoObjetivo: O objetivo deste trabalho foi investigar o efeito doLaser de baixa intensidade (LBI) o sobre macrófagos alveolares(MA) ativado pelo estresse oxidativo e lipopolissacarídeo (LPS). Aterapia com LBI tem sido descrita favoravelmente, pois atua aliviandoos sintomas precoces e tardios de inflamação pulmonar nasvias aéreas. Não se sabe se com o aumento de MIP-2 na expressãodo mRNA e geração de ROS intracelulares observados na inflamaçãopulmonar aguda (IPA) podem ser influenciados pelo LBI. Métodos:Rat AM linha celular, AMJ2-C11 foi cultivado em meio comLPS ou H2O2 e sendo irradiado com Laser ( ë= 660 nm) com Fluênciade 4,5 J/cm2. A produção de MIP-2 mRNA e ROS no MA foramavaliados por Real-Time PCR e 2 ‘, 7’-diacetato dichlorofluorescin(DCFH-DA), respectivamente. A NF- êB proteína na PM foi meartigooriginal - 4.pmd 12/4/2010, 09:23 40 

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