F. Mafra de Lima1M. S. Costa1R. Albertini1J. A. Silva Jr2F. Aimbire11 Institute of Research and Development - IP&D - Av. Shishima Hifumi,2911, Urbanova, 12244-000, São José dos Campos - SP - Brazil.2 Departamento de Ciências da Reabilitação, Centro UniversitárioNove de Julho UNINOVE. Rua Vergueiro, 235 - SP - Brazil.                                                   Abstract                                                                                                                                                                                                                                                                                                                                                                   Background and Objective: It is unknown if the decreasedability to relax airways smooth muscles in asthma and other inflammatorydisorders, such as acute respiratory distress syndrome (ARDS),can be influenced by Low Level Laser Therapy (LLLT) irradiation. Inthis context, the present work was developed in order to investigate ifLLLT could reduce dysfunction in inflamed bronchi smooth muscles(BSM) in rats.                                                                                                                                                                                                                                                                                                                                                                                                             Study Design/Material and Methods: A controlledex-vivo study was developed where bronchi from Wistar rat weredissected and mounted in an organ bath apparatus with or without aTNF-á.                                                                                                                                                                                                                                                                                                                                                                                                                                                         Results: LLLT administered perpendicularly to a point inthe middle of the dissected bronchi with a wavelength (ë) of 655 nm anda dose of 2.6 J/cm2, partially decreased BSM hyperreactivity to cholinergicagonist, restored BSM relaxation to isoproterenol and reducedthe TNF-á mRNA expression. An NF-KB antagonist (BMS205820)blocked the LLLT effect on dysfunction in inflamed bronchi smoothmuscles. Conclusion: The results obtained in this work indicate thatthe LLLT effect on alterations in responsiveness of airway smoothmuscles observed in TNF-á-induced experimental acute lung inflammationseems to be dependent of NF-êB activation.                                                                                                                                                                                                                                                                                                                                                                                                                                                 Descriptors: Low Level Laser Therapy (LLLT); TNF-á; cAMP; NF-êB;Airway Inflammation (Bronchi), Lung                                                                                                       Resumo                                                                                                                                                                                                                                            Não se sabe se a diminuição da capacidade de relaxar os músculoslisos das vias aéreas na asma brônquica e outras doenças inflamatórias,como a síndrome do desconforto respiratório agudo (SARS ou SARA),pode ser influenciada por meio do Laser de Baixa Intensidade (LBI ouLLLT). Neste contexto, o presente trabalho foi desenvolvido, a fim deinvestigar se LLLT poderia reduzir tal disfunção na musculatura lisa dosbrônquios inflamados (DMLBI ou, simplesmente, BSM), em ratos. Umestudo controlado em vivo foi desenvolvido em brônquios de ratos Wistar,que foram dissecados e colocados em um aparelho de banho de órgãos,com ou sem um TNF- á. LLLT administrado usando comprimento deonda ( ë) de 655 nm e dose = 2,6 J/cm2, perpendicularmente, a um pontono meio dos brônquios dissecados. A diminuição da hiperreatividadedos BSM, parcialmente, para agonista colinérgico, foi restaurada pelorelaxamento BSM para isoproterenol e reduziu a expressão do mRNA deTNF- á. O antagonista do NF- êB bloqueou o efeito do LILT sobre adisfunção da musculatura lisa dos brônquios inflamados. Os resultadosobtidos neste trabalho indicam que o efeito terapêutico do LBI, sobrealterações na capacidade de resposta dos músculos lisos das vias aéreas,geralmente, observadas em TNF- á induzida por inflamação pulmonaraguda experimental, parece ser dependente da ativação de NF- êB.                                                                                                                                                                                                                                                                                                                                                                                           Descritores: Terapia com Laser de Baixa Intensidade (LBI), TNF- á,AMPc, NF- êB, Inflamação das Vias Aéreas (Brônquios), Pulmão

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