F. AimbireA. P. Ligeiro de Oliveira2R. Albertini1J. C. Corrêa3C.B. Ladeira de Campos1J. P. Lyon1J. A. Silva Jr4M. S. Costa11 Instituto de Pesquisa & Desenvolvimento - IP&D, Universidade do Valedo Paraíba - UNIVAP. Av. Shishima Hifumi, 2911, CEP: 12244-000. SãoJosé dos Campos - SP - Brazil.2 Laboratório de Farmacologia das Vias Aéreas e Endócrina, Departamentode Farmacologia, Universidade de São Paulo - USP. Av. Prof. LineuPrestes, 1524, CEP: 05508-900. São Paulo - SP - Brazil.3 Departamento de Fisioterapia, Centro Universitário Nove de JulhoUNINOVE. Rua Vergueiro, 235, São Paulo - SP - Brazil.4 Departamento de Ciências da Reabilitação, Centro Universitário Nove

de Julho UNINOVE. Rua Vergueiro, 235, São Paulo - SP - Brazil.

Abstract                                                                                                                                                                                                                                      Background and Objective: Low level laser therapy (LLLT)is a known anti-inflammatory therapy. Herein we studied the effect ofLLLT on lung permeability and the IL-1â level in LPS-induced pulmonaryinflammation.                                                                                                                                                                                                                                                                                                                                          Study Design/Methodology: Rats were dividedinto 12 groups (n=7 for each group). Lung permeability was measuredby quantifying extravasated albumin concentration in lung homogenate,inflammatory cells influx was determined by myeloperoxidaseactivity, IL-1â in BAL was determined by ELISA and IL-1â mRNAexpression in trachea was evaluated by RT-PCR. The rats were irradiatedon the skin over the upper bronchus at the site of tracheotomy afterLPS.                                                                                                                                                                                                                                                                                                                                                                       Results: LLLT attenuated lung permeability. In addition, therewas reduced neutrophil influx, myeloperoxidase activity and both IL-1âin BAL and IL-1â mRNA expression in trachea obtained from animalssubjected to LPS-induced inflammation.                                                                                                                                                                                                                                                                                                                                                             Conclusion: LLLT reducedthe lung permeability by a mechanism in which the IL-1â seems to havean important role.                                                                                                                                                                                                                                                                                                                                                                         Descriptors: Laser Therapy (LLLT), Inflammation, Lung Permeability,Bronchoalveolar Lavage (BAL), Interleukin-1â (IL-1â), Lipopolysaccharide(LPS)Resumo                                                                                                                                                                                                                                                                                                    O Laser de baixa intensidade (LBI) é conhecido por sua açãoanti-inflamatória. Nós estudamos o efeito da LLLT na permeabilidadepulmonar e da IL-1 â nível em LPS-induced inflamação pulmonar.Os ratos foram divididos em 12 grupos (n = 7 para cada grupo). Apermeabilidade pulmonar foi avaliada através da concentração dealbumina extravasada em homogenado de pulmão e o influxo decélulas inflamatórias foi determinado através da atividade demieloproxidase. A IL-1 no lavado broncoalveolar foi analisada porELISA e na traquéia foi avaliada pelo RT-PCR. Os ratos foram irradiadospor sobre a pele na região do brônquio superior na altura datraqueostomia após instilação de LPS. O LBI atenuou o edema pulmonar,além disso, houve redução da migração de neutrófilos, daatividade de mieloperoxidase, e redução dos níveis de IL-1 â no LBAe na traquéia obtidos de animais submetidos à inflamação induzidapor LPS. LBI reduziu a permeabilidade pulmonar por um mecanismoem que a IL-1 â parece ter um papel importante.                                                                                                                                                                                                                                                                                                                                                                                                                                        Descritores: Laser Terapia (LBI), Inflamação, Permeabilidade Pulmonar,Lavado Broncoalveolar (BAL), Interleucina-1 â (IL-1 â), Lipopolissacarídeo(LPS)artigo original - 3.pmd 12/4/2010, 09:23 30 

Este artigo foi postado em 27 de Julho de 2010 às 16:02 e foi colocado sob a categoria Uncategorized. Você pode acompanhar quaiquer respostas a este artigo em seu feed RSS 2.0. Você pode deixar um comentário, ou um trackback do seu site.